Purkan (Dr., S.Si., M.Si.)

Fakultas Sains dan Teknologi, Kimia

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Profil Dosen
Jenjang Pendidikan
No Jenjang Pendidikan Institusi Mulai Berakhir
Data tidak tersedia...
Riwayat Mengajar
No Subjek
Data tidak tersedia...
Bidang Riset
No Subjek
Data tidak tersedia...
Publikasi
No Publikasi Tahun
1 Biochemical characterization of a glucoamylase from Saccharomycopsisfibuligera R64. (66/1: 27—32, DOI: 10.2478/s11756-010-0151-2. Section Cellular and Molecular Biology, Biologia) 2011
2 Novel mutations in katG gene of a clinical isolate of isoniazid-resistant Mycobacterium tuberculosis. (Vol 67, Num 1, 41-47, DOI: 10.2478/s11756-011-0162-7. SectionCellular and Molecular Biology, Biologia) 2012
3 Amobilisasi Enzim GlukosaIsomerase di Dalam Sel Stretomycesgriseus dengan Pemanasan Untuk Proses Produksi Fruktosa. (Jurnal Lembaga Penelitian UNAIR-Surabaya.) 1999
4 Amobilisasi Enzim GlukosaI somerase di Dalam Sel Stretomycesgriseus dengan Pemanasan Untuk Proses Produksi Fruktosa. (Jurnal Nusantara Kimia-Yogyakarta) 2000
5 Penggunaan Bentonit Alam Sebagai Matriks Pendukung Amobilisasi Enzim Amilase. (Jurnal Nusantara Kimia-Yogyakarta) 2001
6 Delesi Fragmen DNA Domain b’ Protein DisulfidaIsomerase Ragi Saccharomyces cerevisiae Menggunakan PCR. (Jurnal PBBMI, Bogor) 2001
7 Expression of pdi1-b’ gene in Escherichia coli. (PI 18. Journal of 2nd Indonesian Biotechnology Conference) 2001
8 Ekspresi gen -amilase Endomycopsisfibuligera pada ragi Saccharomyces cerevisiae. (PI 12. Journal of 2nd Indonesian Biotechnology Conference) 2001
9 The b’x Region Of Yeast Protein Disulfide Isomerase Is Not Essential ForSaccharomyces cerevisiae Viability At Normal (30°C) Temperature. (Journal of Indonesian Micro Biology, Jakarta) 2008
10 Mutation of katG Gene In Mycobacterium tuberculosis That Promoting Malfunction of Catalase/Peroxidase Leads to Isoniazid Resistance.. (Journal of Mathematics and Sciences, ITB, (summited)) 2012
11 Exploration of Amylolytic Enzyme : Cloning gene encoding glucoamylase of Endomycopsisfibuligera Rcc64 in Escherichia coli using T vector. (Proceeding of Asean Biochemistry Seminar and Workshop, Surabaya) 2006
12 Amylolytic Enzyme of Endomycopsisfibuligera Rcc64: Amplification of Coding Region of Gene Encoding Glucoamylase using PCR. (Proceeding of Asean Biochemistry Seminar and Workshop, Surabaya) 2006
13 ASEAN Biochemistry Seminar “ Enzyme : industrial and medical prospects”. (UA, Univ. of Groningen and Koninklijke Nederland Akademie van Wetenschappen) 2006
14 International conference on basic and applied sciences. (UA, RuG, KNAW dan UTM) 2006
15 IOCD International Symposium (Biology, Chemistry, Pharmacology and clinical Studies of Asian Plants). (IOCD & Faculty of Pharmacy UA) 2007
16 Mapping of gene Encoding Glucoamylase Endomycopsisfibuliegera ITB.R.cc.64 (GLOI) in T Vector Recombinant. (Proceeding of International Conference and Workshop on Basic and Applied Sciences, Surabaya) 2007
17 Single amino acid residue substitution in two isolates of isoniazid-resistant Mycobacterium tuberculosis catalase-peroxidase. (Proceeding onInternational Conference on Biotechnology : Biotechnology for better life, ITB’S Golden Anniversary., Bandung ) 2009
18 Mutations at both N and C domain of protein KatG could be a factor that increase the level of isoniazid resistance in Mycobacterium tuberculosis. (Proceeding in JSChem UKM-ITB VIII, Bangi, Malaysia) 2009
19 The third International Conference on Mathematics and Natural Sciences (ICMNS). (ITB, Bandung) 2010
Keanggotaan Asosiasi
No Asosiasi
Data tidak tersedia...
Penghargaan
No Penghargaan Tahun
1 The First Winner Microbilogy Indonesia Award 2009 . (Microbilogy Indonesia) 2009
2 Penyaji Poster Terbaik Seminar HasilPenelitianStrategisNasional (SK DP2M-Ditjen Dikti, KemdiknasNomor: 2073/D3/PL/2010). (DP2M-Ditjen Dikti, Kemdiknas) 2010
Informasi Lain

II. EDUCATIONAL BACKGROUND
2.1. Program: Bachelor Master Doctoral
2.2. University UA ITB ITB
2.3. Discipline Kimia Biokimia Biokimia

III. AWARDS
Number Years Name of Awards From
1. 2009 The First Winner Microbilogy Indonesia Award 2009 Microbilogy Indonesia
2. 2010 Penyaji Poster Terbaik Seminar HasilPenelitianStrategisNasional (SK DP2M-Ditjen Dikti, KemdiknasNomor: 2073/D3/PL/2010) DP2M-Ditjen Dikti, Kemdiknas

IV. ACADEMIC SERVICES
Teaching:
1. Subjects (levels)
2. Subjects (levels)
3. Subjects (levels)
4.
(ex. Research Methods (undergraduate, master and doctoral level)

Graduated Advisees:
- Undergraduate: … students
- Master: … students
- Doctoral: … students
Advising:
- Undergraduate: … students
- Master: … students
- Doctoral: … students

V. RESEARCH INTERESTS
…………
VI. RESEARCH EXPERIENCE

No. Years Title Funding
Sources Amount (in Rupiah)
1. 1995 FraksinasiEkstrakKasarAmilasedariEndomycopsisfibuligeraUntukMemisahkanSaccharifyingdan Liquefying Amylase MenggunakanAmoniumSulfat Mandiri (Skripsi)
2. 1997 OptimalisasiproduksienzimglukosaisomerasedariStreptoycesgriseus Dik Rutin UA
SK. Retor No. 5935/JO3/PL/ 1997
3. 1998 Upaya meningkatkan translokasiamilasekelingkunganekstraselulerselama proses fermentasiEndomycopsisfibuligera Proyek Pengkajian dan Penelitian Ilmu Pengetahuan Terapan. Kontrak No. 06/P2IPT/ DPPM/98/LIT-MUT/V/1998
4. 1998 Pembuatan peta restriksi DNA kromosom Endomycopsis fibuligera ITB Rcc 64 DIK Suplemen Unair. SK Rektor No. 5415/JO3/PL/ 1998
5. 1998 Amobilisasi amilase menggunakan bentonit untuk efisiensi produksi glukosa cair bagi berbagai industri Proyek Pengkajian dan Penelitian Ilmu Pengetahuan Terapan. Kontrak No. 43/P2IPT/ DPPM/98/PHB VI/2/1998
6. 1999 Isolasi, pemurnian dan karakterisasi enzim glukosa isomerase dari streptomyces griseus Proyek Pengkajian dan Penelitian Ilmu Pengetahuan Terapan. Kontrak No. 022/P2IPT/ DPPM/98/LIT-MUT/V/1999
7. 2001 KonstruksidanKarakterisasiMutan Protein DisulfidaIsomeraseRagi Yang TidakMengandung Domain b’ Mandiri (Tesis)
8. 2003 Kloning Dan Over Ekspresi Gen Protein DisulfidaIsomeraseRagiSacharomycesCerevisiae Di Escherichia Coli ProyekPeningkatanPenelitianPendidi-kanTinggi
DIP Nomor : 006/XXIII/ 1/--/2003
9. 2004 Produksi, PemurnianParsial Dan KarakterisasiEnzimManosaIsomerase Dari StreptomucesGriseus.
DIK RutinUnairtahun 2004 SK RektorUnair No. 4222/J03/ PG/2004

10. 2005 & 2006 KonstruksiRagi (Saccharomyces Cerevisiae) Rekombinan Yang MampuMencernaPatiMelaluiKloning Gen PenyandiEnzimGlukoamilase.
(Laporan Penelitian Hibah Bersaing Tahun Anggaran 2005) HibahBersaingProyek DP3M, DiktiDepdiknas No. kontrak : 746/J03.2/ PG/2005 & No. kontrak: 318/SP3/PP/DP2M/II/ 2006
11. 2008 Construction of Yeast Recombinant (Saccharomyces cerevisiae)Having Glucoamylase Activity For Direct AlchoholFermentation of Starch DIPA Biotrop 2008
12. 2009 MekanismeResistensi Isoniazid PadaMicobacterium tuberculosis : AnalisisMutasi Gen katGdanKaitannyaDenganSifatBiokimiaEnzimKatalase-PeroksidaseMutanRekombinan Stranas-DIPA Unair 2009
Nomor: 276/H3/KR/2009
12. 2008-2009 Redisaineksplorasimikrobapenghasilglukanasebarupenghidrolisisplakgigisebagaipencegahgigidanpeiodontitis Ristek RD-2009-991
13. 2011 Aktivitas Katalitik Mutan Protein KatG Isolat Klinis Mycobacterium tuberculosis Dan Kaitannya Dengan Resistensi Isoniazid
RisbinIptekdok 2011
14. 2011 Mutan katG dari Mycobacterium tuberculosis isolat klinis dan karakterisasi produk gennya Mandiri (Disertasi)

VII. PUBLIC SERVICES EXPERIENCES
No. Years Title Funding
Sources Amount (in Rupiah)
1. 200../200..

VIII. ACADEMIC PUBLICATIONS
No. Year Title Volume (Issue) Journals
A. International Journals – Published
1. 2011 Biochemical characterization of a glucoamylase from Saccharomycopsisfibuligera R64. 66/1: 27—32, DOI: 10.2478/s11756-010-0151-2 Section Cellular and Molecular Biology, Biologia
2. 2012 Novel mutations in katG gene of a clinical isolate of isoniazid-resistant Mycobacterium tuberculosis Vol 67, Num 1, 41-47, DOI: 10.2478/s11756-011-0162-7 SectionCellular and Molecular Biology, Biologia.

B. National Journals – Published
1. 1999 OptimalisasiProduksiGlukosaIsomerasedari Streptomyces griseus 1. JurnalLembagaPenelitianUNAIR-Surabaya.
2. 2000 AmobilisasiEnzimGlukosaIsomerase di DalamSelStretomycesgriseusdenganPemanasanUntuk Proses ProduksiFruktosa JurnalNusantaraKimia-Yogyakarta
3. 2001 PenggunaanBentonitAlamSebagaiMatriksPendukungAmobilisasiEnzimAmilase JurnalNusantaraKimia-Yogyakarta
4. 2001 DelesiFragmen DNA Domain b’ Protein DisulfidaIsomeraseRagi Saccharomyces cerevisiaeMenggunakan PCR Jurnal PBBMI, Bogor
5. 2001 Expression of pdi1-b’ gene in Escherichia coli PI 18 Journal of 2nd Indonesian Biotechnology Conference
6. 2001 Ekspresi gen -amilaseEndomycopsisfibuligerapadaragiSaccharomyces cerevisiae PI 12 Journal of 2nd Indonesian Biotechnology Conference
7. 2008 The b’x Region Of Yeast Protein Disulfide Isomerase Is Not Essential ForSaccharomyces cerevisiae Viability At Normal (30°C) Temperature. Journal of Indonesian Micro Biology, Jakarta.
8. 2012 Mutation of katG Gene In Mycobacterium tuberculosis That Promoting Malfunction of Catalase/Peroxidase Leads to Isoniazid Resistance. Journal of Mathematics and Sciences, ITB, (summited)

C. Under Review
1.

D. International Conferences
1 2006 Exploration of Amylolytic Enzyme : Cloning gene encoding glucoamylase of Endomycopsisfibuligera Rcc64 in Escherichia coli using T vector Proceeding of Asean Biochemistry Seminar and Workshop, Surabaya
2. 2006 Amylolytic Enzyme of Endomycopsisfibuligera Rcc64: Amplification of Coding Region of Gene Encoding Glucoamylase using PCR Proceeding of Asean Biochemistry Seminar and Workshop, Surabaya
3. 2006 ASEAN Biochemistry Seminar “ Enzyme : industrial and medical prospects” UA, Univ. of Groningen and Koninklijke Nederland Akademie van Wetenschappen
4. 2006 International conference on basic and applied sciences UA, RuG, KNAW dan UTM
5. 2007 IOCD International Symposium (Biology, Chemistry, Pharmacology and clinical Studies of Asian Plants) IOCD & Faculty of Pharmacy UA
6. 2007 Mapping of gene Encoding GlucoamylaseEndomycopsisfibuliegera ITB.R.cc.64 (GLOI) in T Vector Recombinant Proceeding of International Conference and Workshop on Basic and Applied Sciences, Surabaya
7. 2009 Single amino acid residue substitution in two isolates of isoniazid-resistant Mycobacterium tuberculosis catalase-peroxidase Proceeding onInternational Conference on Biotechnology : Biotechnology for better life, ITB’S Golden Anniversary., Bandung
8. 2009 Mutations at both N and C domain of protein KatG could be a factor that increase the level of isoniazid resistance in Mycobacterium tuberculosis Proceeding in JSChem UKM-ITB VIII, Bangi, Malaysia
9. 2010 The third International Conference on Mathematics and Natural Sciences (ICMNS) ITB, Bandung

IX. MEMBERSHIP
1. (Ex. American Marketing Association, since 2009)
2. …
3. …
X. RESEARCH SERVICES

 

XI. BOOKS
No. Year Title Number of Pages Publisher
1.
2.

Kontak Dosen

purkan@unair.ac.id
031 5922427
-
Kampus C Mulyorejo Surabaya Indonesia